The recent Corona Virus, which is known as the Covid-19 virus, first emerged in December 2019 and rapidly spread to all parts of the world, causing a global pandemic. The high number of cases of the virus in all countries of the world and its associated mortality has become a source of great concern and leading to a number of studies being conducted to evaluate what will help control the spread.
The newly discovered RNA-guided genome editing system known as CRISPR-Cas9 allows efficient and precise targeting of specific genes and offered numerous benefits over the previously used Gene Therapy systems.
Gene Therapy
Gene Therapy is a system that involves alteration of the DNA or RNA of an organism to facilitate therapeutic uses. It can be used for the treatment or preventative of…
Definition:Genome editing (also known as genome engineering or gene editing) was first pioneered in 1990 and defined as:
‘a technique used for modification of DNA within a cell by cutting at particular sequence by an enzyme (engineered nuclease), which leads to a change in physical traits.’
This technological advancement allows genetic material to be added, modified, or…
Current Realities of Gene Editing:
Gene editing technologies, specifically CRISPR-Cas9, are intensively progressing in clinical trials, covering all biological sciences from biotechnology to medicine. The clinical uses are limited to ex vivo gene editing and then putting back inpatient. Gene editing is also implacable to those diseases which require cell modification in vivo.
Genome…
CRISPR or Cas9 is short for clustered regularly interspaced short palindrome repeats. This is a unique technology that allows geneticists and medical scientists to edit genome components by removing, adding, or modifying DNA sections. In the scientific community, the CRISPR-Cas9 system has generated optimism due to its simplicity of use, and is generally more precise and effective than other…
Short variable sequences called spacers are interspersed between the short DNA repeats of bacterial CRISPRs. These spacers are collected from the DNA of viruses that have invaded the host bacterium before. Spacers, therefore, function as a ‘genetic memory’ of past infections. If there is another infection caused by the same virus, any viral DNA sequence matching the spacer sequence…
An unusual pattern of DNA sequences in a gene belonging to Escherichia coli, a microbe that lives in the intestine, was discovered by a Japanese team of scientists at Osaka University in 1987. The gene tended to have five short repeated fragments of DNA divided by short ‘spacer’ DNA sequences that did not repeat. The five repeated fragments had similar sequences, the building blocks of…
Gene editing tools have evolved over the years, leading to the current rise in their popularity. We will discuss how gene editing works and describe different techniques that can be used. ZFN, most commonly based on the FokI restriction enzyme, is the oldest and most recognized of the DNA-binding proteins, fused to a zinc finger’s DNA-binding domain designed to target a specific DNA…
CRISPR Treatment is very effective in gene editing. CRISPR-Cas9 has been proven to benefit human beings in treating major genetic defects. The protein CAS9 Acts as a molecular scissor that can cut the defected DNA strands (Crawford, 2017). Gene editing has been made simple and easy by the CRISPR treatment. Many people are getting various health benefits by using this significant…
CRISPR technology is becoming more widely used each year. It is a powerful tool to treat many gene defects. It has made the editing of genomes quite simple and easy. Gene editing is very important in treating various genetic disorders.
CRISPR-Cas9 are specialized strands of DNA. There is a protein that we call Cas9, which acts like a scissor that cuts the strands of DNA causing genetic…